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Description
Human MT-1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a metallothionein 1 (MT-1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of metallothionein 1 (MT-1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Metallothionein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Metallothioneins are a family of cysteine-rich, low-molecular-weight proteins (MW ranging from 500 to 14,000 Da). They are localized to the membranes of the Golgi apparatus. MTs have the ability to bind physiological (such as zinc, copper, and selenium) and xenobiotic (such as cadmium, mercury, silver, and arsenic) heavy metals via thiol groups on their cysteine residues, which comprise approximately 30% of their amino acid composition. Four major isoforms are expressed in humans: MT1 (isoforms A, B, E, F, G, H, L, M, and X), MT2, MT3, and MT4. In humans, they are synthesized in large quantities primarily in the liver and kidneys. Their production depends on the dietary availability of minerals such as zinc, copper, and selenium, as well as the amino acids histidine and cysteine. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.8 ★★★★★
Based on 1819 reviews
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Product Reviews
★★★★★ 4
Comfortable, easy to assemble, but no adjustable armrests
Color: Dark, Color: Dark
The Marsail Executive Office Chair Ergonomic Desk Chair with Flip-up Armrests PU Leather was an easy to assemble chair; I was done within 15 minutes with the included tool. Extra screws were included which was nice as the main pack had broken open and I couldn't find one of the primary screws in the larger box (my guess is I probably tossed it on accident with the packaging material) but had the backup so all was well. The instructions were well written and clear.
I've now had the chair in my office for over a week. For context; I work from home and also am a PC gamer so I spend a sizeable portion of my time at my desk in the chair. I also live in the southeast US and its April when I am writing this review. With that said; its been in the 70s/80s regularly so feel like I can comment fairly on its performance in warmer temps; though I'll have to try and remember to comeback and update it for the summer season when AC is on full blast and humidity is higher (and the inevitable "swamp butt" sets in).
In the first week I've found the chair to be comfortable to sit in. It is wide enough and the arm rests to the side enough that I can sort of lean left and right without much trouble. I like this wider seat and armrests compared to chairs with more narrow span as I tend to shift and move a lot while sitting.
The PU leather material feels nice against my clothes and does "grip" the clothing which helps with my tendency to want to slide forward when sitting. At first I thought the "grip" would be annoying but I sort of like it after a week of using the chair.
The cushion material is comfortable for sitting and rebounds well after I've been sitting in it for a long period of time. The armrests feature the same sort of cushion and PU leather material which makes them comfortable to sit back and lean on.
For the price I think this is a solid office chair and a comfortable option. Longevity will only be tested over time so I'll see if I can remember to update this review over time. I am happy with the chair in my office.
A few downsides about the chair that I have:
- The wheels are very stiff and don't roll as smoothly as I'd like. They stay put when I am sitting at the desk but when I need to push away they take a bit of force.
- The arm rests can't be adjusted for height. They are one height and can only be moved out of the way completely. For someone like who likes to lean or shift sides this is less than ideal as I'd normally make adjustments to the armrests. With that said it is nice to be able to move them out of the way completely when I don't need them.
- Lumbar support could be better. I will be using additional support in the chair for my back.
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Reviewed in the United States on April 13, 2026
★★★★★ 5
Good for sitting on it daily. Not that soft as expected
Color: Dark, Color: Dark
I’ve been using this chair for a few days now and overall it’s pretty solid for a home office setup, especially for the price range. Assembly took me about 30–40 minutes alone and all the parts were labeled clearly. Nothing arrived damaged and the instructions were easy to follow, though attaching the backrest was a little awkward with one person.
Once assembled, the chair looks clean and professional. The PU leather feels smooth and the cushioning is definitely thicker than cheaper office chairs I’ve tried. It’s comfortable for working several hours at a time, although I wouldn’t describe it as super plush or “sink-in” soft. The seat has a firmer feel, which may actually be better for posture during long work sessions.
The ergonomic support is decent. The backrest follows the shape of your back fairly well and gives noticeable lumbar support compared to flat office chairs. I spend a lot of time at my desk and this chair helped reduce some lower back discomfort. The reclining and rocking feature is also nice for quick breaks between meetings or while watching videos. It doesn’t recline extremely far, but enough to relax a bit without feeling unstable.
One feature I ended up liking more than expected was the flip-up armrests. They make it easy to slide the chair completely under the desk and also give more room if you like sitting cross-legged occasionally. The chair rolls smoothly and feels stable even when leaning back.
A couple downsides though. The faux leather can get warm after extended sitting, especially in a warmer room. The mesh section helps airflow a little, but not enough to fully prevent heat buildup. Also, while the chair is comfortable, it doesn’t quite feel “premium executive” level in terms of materials. Some of the plastic components and armrests feel more mid-range than luxury.
Overall, I’d say it’s a good value office chair with solid comfort, decent back support, and practical features. Great for everyday work or study use, just keep expectations realistic if you’re looking for a super high-end executive chair feel.
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Reviewed in the United States on May 6, 2026
★★★★★ 5
Comfortable chair constructed with good quality materials
Color: Dark, Color: Dark
Having purchased several chairs from Amazon over the years, I would rank the Marsail Executive Office Chair Ergonomic Desk Chair as one of the better quality options I've received. Here is a breakdown of my experience:
Build Quality & Aesthetics
I was immediately impressed by the quality of the base. It is crafted from brushed metal and has a substantial, premium feel that sets it apart from cheaper plastic alternatives. Additionally, the pneumatic height adjuster was easy to install and operates very smoothly.
Comfort & Ergonomics
-- Padding: The padding is not quite as thick as I expected, but it has proven to be very comfortable to sit in so far. Sometimes I am in the chair as much as 10 hours per day, and the foam in the seat holds it's form all day.
-- Support: The chair does not include built-in lumbar support, so I am using a lumbar pillow. However, I actually find this preferable; it allows me to adjust the pillow to my exact needs or remove it entirely depending on how I'm feeling.
-- Head support and tilt: I like to work in an upright position, but I sometimes i like to lean back for a rest. The chair leans back just enough to allow me to rest my head, but not so much that I feel like it will tip over. The head support is subtle too - it doesn't hurt my neck or force me to rest in an awkward position.
Functionality
-- The mobility of this chair is excellent. The wheels are high-quality and roll very smoothly on my hardwood floors, which is often a pain point with other office furniture.
Final Verdict
Despite the thinner padding and lack of integrated lumbar support, the superior materials and smooth mechanics make this a decent value for the money. It’s a sturdy, well-made piece of furniture.
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Reviewed in the United States on April 12, 2026
★★★★★ 4
Good quality office chair with movable arms
Color: Dark, Color: Dark
This chair was relatively easy to put together. After unboxing and laying out all of the parts, my husband and I built it in 22 minutes. We noticed how the instructions have you put the tilt mechanism bracket on after putting the back and arms on. Another chair we built was the opposite, which made attaching it to the base actually easier. That way just made more sense.
This way, you put the back and seat together using the arms, then you attach the tilt mechanism to the bottom of the seat, but the seat is at an angle when you do this. Then you lift the whole chair and place it down onto the legs. I'm glad there were two of us to do that part. We also noticed one of the holes for screwing in the arm bracket was not lined in metal like the rest of them were. It still worked, though.
It does rock a bit back and forth. The height adjusting rod also locks the chair in place for when you don't want the chair to lean back. There is a knob underneath that allows you to loosen or tighten how much it moves when you lean back or rock.
The material is faux leather. It looks like real leather and feels pretty good. This makes it easy to clean. The arms can be raised level with the back of the chair to make it easy to slide out of the side of the chair. Overall this chair has a pretty classic look to it and is comfortable to sit in. It even has some lumbar support.
The way the back of the chair and the seat of the chair connect together leaves a little bit of a gap. Nothing major, but you could lose coins or keys through the gaps between the cushions. If you have a cat, this would be a great way for them to poke their paws through and say hello.
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Reviewed in the United States on May 20, 2026
★★★★★ 5
EXCELLENT choice for the money!!!
Color: White, Number of Items: 1
I have never written a chair review, however; I feel this product warrants it.
I got this chair to replace an old chair in my studio. I scoured Amazon for hours to find it, and I am glad that I took the time to do so!
I was worried about material quality and durability at this price point, but my expectations were FAR exceeded. The piece goes together with a quickness (for me, under 10 minutes), and I only glanced at the instructions once.
Suffice to say, setup is simple and intuitive. Once the chair is together, there is a slight amount of play where the base plate attaches to the shaft, however it isn't a ridiculous amount. I only noticed as I was examining it and being critical after my initial excitement.
The materials used are very good, and much better than you would likely get from other chairs at this price. The chrome finish is nice, and the chair PU is uniform color with no defects.
One really nice feature are the zippered arm rest covers; when they get dirty, which (being white) they eventually will, you can unzip them, lay them flat, and clean them laid out, as opposed to struggling to scrub around 90 degree angles. I am sure this was easier for the manufacturer, but the end result helps the end user as well. Good on them for that.
Another thing I am very happy with is the height; I am 6'4" in bare feet, and my wife is 5'0". We both fit comfortably after adjusting. They definitely got the seat height right. And to that point (of size), at my height, and 260 pounds, there is plenty of room at the sides. Mind you, however, I am fit. That is not to say that a 5'10" guy that is 260 will find it comfortable. He may not. That said, most people should fit in this without an issue.
The casters had a positive snapping-in, and roll over my low carpet easily. This chair is definitely well built, save for the slight play mentioned earlier. In no way does that detract from it at all. The aesthetic fits my studio perfectly, and in all, I would not hesitate to recommend this chair.
If I have any issues with it, I will post an update review, but so far so good. I really love it. If you like the way it looks, pull the trigger. It is definitely worth the price, and more.
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Reviewed in the United States on February 6, 2021
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