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Description
Human MG7-Ag ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Preparation of standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 15 U/mL). Then dilute to the following concentrations: 15 U/mL, 7.5 U/mL, 3.75 U/mL, 1.875 U/mL, 0.9375 U/mL, 0.46875 U/mL, 0.234375 U/mL, 0.000 U/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 15U/mL standard working solution into the first EP tube and mix thoroughly to make a 7.5U/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with MG7-Ag capture antibody. After incubation and washing, the sample is developed with the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of MG7-Ag in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human MG7-Ag ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Gastric cancer antigen (MG7-Ag) is a highly specific antigen associated with gastric cancer. Its expression in gastric cancer tissues has a relative histological type bias. The morphological, biochemical, and antigenic changes that occur in gastric mucosal epithelial cells during carcinogenesis are related to genetic damage and dysregulation. Due to different genetic changes, different types of gastric cancer have different antigen expression sequences and groups. In well-differentiated adenocarcinomas, cells are well differentiated and have minimal atypia. Antigens are primarily found on the cell membrane and microvilli of the glandular cavity, with very little in the cytoplasm. In poorly differentiated adenocarcinomas, antigens are found in both the cell membrane and cytoplasm, with a scattered distribution. Antigens are also found on the Golgi complex, rough endoplasmic reticulum, and nuclear membrane in poorly differentiated adenocarcinomas. In undifferentiated adenocarcinomas, only small amounts of antigenic material are present in the cytoplasm. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.2-15 U/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.9 ★★★★★
Based on 1127 reviews
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Product Reviews
★★★★★ 5
🦴 Micro Bone, Major Relief; Perfect Puppy Teething Chew Toy
Size: X-Small, Color: Blue, Size: X-Small, Color: Blue
🐾 Hi, I’m PVD; teething survivor, nub enthusiast, and gum massage advocate.
This tiny blue bone was my day-one favorite from 8 weeks to 5.5 months, it handled every chew session, tantrum, and tooth eruption with dignity. I’ve officially “aged out,” but I still salute it.
⭐ Why it earned 5 stars:
✔️ Perfect XS size for toy breed jaws
✔️ Soft on sore gums, durable enough for dramatic chewing
✔️ Ridges + nubs = mouth spa treatment
✔️ Chilling it = elite teething relief
✔️ Kept me busy and prevented chewing on illegal things (furniture, shoes… mother)
🌀 Why I’m now gracefully retiring it (but still recommending):
✘ Jaw strength and confidence grew but I didn’t vibe with the larger size versions (tried and rejected them with judgment)
✘ Texture now too gentle for my post-teething chew style
✘ Advanced chewers may get bored once the rage-chewing era passes
✘ I get gum relief via Nylabone Nubz edible chews now
Best For:
• XS-Small breed puppies, especially under 5 lbs
• Teething stages + gum relief
• Delicate chewers or fur babies still in the love-bite phase
• First-time puppy parents (trust me, this bone prevents household drama!)
• 8 weeks–6 months old
🚫 Not for adult teeth or advanced chew techniques.
📝 Final Re-Barks:
Iconic starter bone for toy breed pups. Affordable, safe, and genuinely soothing during teething chaos. It carried me through the hardest months (and saved several legs; both human and furniture). I’ve outgrown it; but I’ll forever endorse it for the next generation of tiny chewers.
— PVD (Tiny Tooth Graduate, Former Chew Therapy Success Story 🧊🦴)
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 17, 2025
★★★★★ 3
Nylabone Puppy Treat Starter Pack - A Mixed Bag of Delight and Disappointment
Size: Small, Color: Blue
I recently purchased the Nylabone Puppy Treat Starter Pack for my adorable 5lb puppy, and overall, I have mixed feelings about this product. While I was initially satisfied with the variety pack, one of the bones in particular left me disappointed due to its lack of durability.
Let's start with the positives. The Nylabone Puppy Treat Starter Pack comes with three different toys: a blue puppy teething toy, a nylon dog toy, and a chew treat. This variety is great for keeping my puppy engaged and entertained, especially during the teething phase. The blue puppy teething toy is specifically designed for soothing sore gums, and my puppy seemed to find great relief when chewing on it. It has a pleasant texture that is gentle on the teeth and gums, making it an ideal choice for teething puppies.
The nylon dog toy included in the pack is durable and sturdy. It has a satisfyingly textured surface that provides an excellent chewing experience for my little furry friend. The toy is designed to withstand the strong chewing habits of puppies, and it has certainly proven to be long-lasting compared to other toys I've tried in the past. My puppy enjoys gnawing on it for extended periods without any signs of damage.
Now, let's address the bone that failed to meet expectations. One of the bones included in the pack was devoured by my puppy within a single evening. I was taken aback by how quickly it was consumed, especially considering my puppy's small size. While the other bones in the pack held up well, this particular one lacked the durability I had hoped for. I understand that puppies have strong jaws, but I expected the bone to withstand more than a few hours of chewing.
Despite this disappointment, I must mention that the bone did not cause any harm to my puppy. It is important to supervise your puppy while they chew on any toys, especially if they have a tendency to swallow large chunks. In this case, I was fortunate that my puppy did not experience any adverse effects after consuming the bone. However, it's crucial to exercise caution and ensure that your puppy doesn't ingest anything that could potentially be harmful.
Overall, while the Nylabone Puppy Treat Starter Pack has its upsides, I cannot ignore the fact that one of the bones failed to meet expectations in terms of durability. However, the other toys included in the pack provided a satisfactory experience for my puppy, and they have proven to be long-lasting. I would recommend this product with the caveat that you closely monitor your puppy's chewing habits and potentially avoid the bone that didn't hold up well in my particular case.
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Reviewed in the United States on May 31, 2023
★★★★★ 5
⭐⭐⭐⭐⭐ Great for teething puppies
Size: Small, Color: Pink
I purchased the Nylabone puppy chew toys for my teething puppy, and they’ve been a big help. The toys are the perfect size for a young puppy and feel durable without being too hard.
My puppy enjoys chewing on them, and they’ve helped redirect chewing away from furniture and shoes. I also like that they’re designed specifically for puppies, so they’re gentle enough for growing teeth and gums.
The variety in the set keeps my puppy interested, and the toys have held up well with daily use. They’re easy to clean and feel safe to give to a puppy.
Overall, these are great chew toys for puppies. They’re effective, well made, and helpful during the teething stage. I would definitely recommend them to new puppy owners.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 15, 2025
★★★★★ 5
Happy puppy with this durable bone
Size: X-Small, Color: Pink
Our new puppy loves the texture and thickness of this bone. She is an aggressive chewed and has not yet destroyed this bone. We have purchased Nylabones for our dogs year after year and have had several happy puppies over the years.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 4, 2026
★★★★★ 5
Great Variety for Teething Puppies, Keeps Him Busy!
Color: 9 Pack (No-stuffed Fox), Color: 9 Pack (No-stuffed Fox)
I got this 9-pack toy set for my 11 lb chihuahua-terrier mix puppy, and it’s been very well received by my little guy. He’s been bored being in quarantine until his vaccines kick in and he's also teething. This toy set has made a huge difference.
Great variety - you get a combination of rope toys, plush squeaky toys, and even a treat ball, so he often rotates between them depending on what he wants. The rope toys have been especially good for teething, while the softer toys keep him entertained. One of the plush toys has that crinkly texture inside, which he loves.
The treat ball is a nice bonus too. I've put treats and his dog kibble in there throughout the day, and it helps keep him engaged and also mentally tires him out.
Everything is sized well for a small puppy and light enough for him to carry around easily.
The materials feel decent for the price, especially considering how many toys you get. That said, I do keep an eye on him with the softer ones since he’s still a puppy and likes to chew.
Pros:
Good mix of rope, plush, and interactive toys
Great for teething and keeping him occupied
Treat ball adds a mental challenge
Good size for small dogs/puppies
Nice value for the number of toys
Cons:
Softer toys may wear down faster with heavier chewing
Overall, this has been a really useful set during a phase where he’s both teething and needs a lot of mental stimulation. I’d recommend it for small dogs or puppies, especially if you want a variety instead of buying individual toys.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 14, 2026
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