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Description
Human PPIC ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Peptidyl-prolyl cis-trans isomerase C (PPIC). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Peptidyl-prolyl cis-trans isomerase C (PPIC) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Peptidyl-prolyl cis-trans isomerase C ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Peptidylprolyl isomerase C (PPIC), also known as CYPC or Cyclophilin C, is an enzyme encoded by the PPIC gene. As a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family, this protein catalyzes the cis-trans isomerization of the proline-imidazole peptide bond, enabling it to facilitate protein folding or repair. Furthermore, it is involved in numerous biological processes, including mitochondrial metabolism, apoptosis, redox reactions, and inflammation, as well as related diseases and conditions such as ischemia-reperfusion injury, HIV/AIDS, and cancer. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.8 ★★★★★
Based on 1711 reviews
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Product Reviews
★★★★★ 5
Adds comfort and support
Color: Green, Size: Twin
I’ve been using this bamboo mattress topper for a couple of weeks now, and it completely changed the feel of my bed. My mattress was starting to feel too firm and uncomfortable, especially on my lower back and shoulders, but this topper added the perfect amount of cushioning without making the bed feel overly soft or saggy.
The cooling effect was one of the biggest surprises. I tend to sleep hot and usually wake up sweating during the night, but the bamboo-derived fabric feels breathable and much cooler than regular mattress pads I’ve owned before. It stays comfortable throughout the night and doesn’t trap heat like memory foam toppers sometimes do.
I also really appreciate the waterproof protection. It gives peace of mind without feeling stiff or making that annoying crinkling noise some waterproof covers have. The surface still feels soft, plush, and smooth. It honestly feels more like a hotel-style pillow top than a basic mattress pad.
The thickness is just right for pressure relief. It helped reduce the soreness I usually wake up with in my back, and I’ve been sleeping much more comfortably overall. The deep pockets also held securely on my twin mattress and didn’t slide around, even after moving a lot during sleep.
Another thing I liked was how fluffy and well-made it looked right out of the package. After letting it expand for a bit, it became nicely plush and evenly padded. The stitching seems durable, and it still looks great after washing.
If you’re looking for a softer, cooler sleeping surface with added waterproof protection and better support for aches and pains, this mattress topper is definitely worth it. It made my bed feel newer and far more comfortable without needing to buy a whole new mattress.
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Reviewed in the United States on May 14, 2026
★★★★★ 5
BDEUS Twin Mattress Topper, So Soft
Color: Green, Size: Twin, Color: Green, Size: Twin
Having purchased BDEUS products in the past, I was very looking forward to ordering the mattress topper made of viscose (a material derived from bamboo) just to see (and feel) the benefits of the material.
My concern about the material, viscose derived from bamboo, is that the process to manufacture the material can be chemically intensive leading to harsh smells. I can say the topper has no off-putting smells. The quality of the manufacturing is present as there are no loose threads/seams, the material has no defects, and the print is uniform. The use of the material also leads to increased durability while enhancing the topper’s breathability and cooling properties.
The topper is fitted meaning the topper will not come off the mattress very easily (even though you may need sheet straps if you are a wild sleeper). But the softness is wild, I mean this has to be one of the softest materials I have felt, and it feels very luxurious. Waterproofing and moisture-wicking is also a benefit as small spills are not absorbed into the fabric.
The topper is also very thick and plush, and while the product listing touts “Back Pain Relief” it may be too soft depending on how firm, or soft, you prefer your mattress. I have to say this is definitely a five-star offering from BDEUS. This topper is for my daughter but I’m going to order a queen-sized topper for my bed as the price is just right.
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Reviewed in the United States on May 14, 2026
★★★★★ 4
Definitely not cooling but comfortable
Color: Dark Grey, Size: Queen
Definitely makes my mattress more comfortable. Not finding it very cooling! Came quickly and arrived intact
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Reviewed in the United States on May 20, 2026
★★★★★ 5
This topper has definitely provided me with better, more comfortable sleep.
Color: Green, Size: Twin
I recently tried a twin mattress topper made from bamboo-derived viscose, and I'm quite impressed. The topper is thick, waterproof, and offers excellent comfort, especially for those who struggle with back pain or sleep hot. The bamboo and polyester blend gives it a soft, cooling surface that's perfect for a restful night's sleep without the sweaty feeling often associated with memory foam.
What I really appreciate is its dual function. It's breathable, allowing for good air flow, and fully waterproof, which helps keep the mattress underneath safe from spills. This is especially useful if you have kids or pets.
The topper's thickness is just right—it provides great support without feeling too soft or too firm. It cradles pressure points well, offering relief to my hips, back, and shoulders.
The fluffy filling holds up well over time, maintaining its softness and support. It fits securely on mattresses, thanks to a deep pocket skirt that prevents any sliding or bunching. This topper has definitely provided me with better, more comfortable sleep.
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Soft and comfy
Color: Green, Size: Twin, Color: Green, Size: Twin
This mattress pad is much thicker and fluffier than the one I had before. With I took it out of the package, it was vacuum sealed with a note saying to leave it out in the sun for 48-72 hours or fluff it up with a hair dryer. I put it in the dryer on medium heat, which worked nicely. The pockets are really deep on this mattress pad and it fits snug. You don’t really see the shape with a sheet on top. It feels comfortable laying on top of it. It doesn’t flatten much when I’m laying down, but I imagine over time it will lose fluffiness. Overall it’s comfortable and breathable.
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Reviewed in the United States on May 31, 2026