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Description
Human HDAC1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a histone deacetylase 1 (HDAC1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of histone deacetylase 1 (HDAC1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Histone Deacetylase 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Histone deacetylase 1 (HDAC1) is an enzyme encoded by the HDAC1 gene. Diseases associated with HDAC1 include Atrichia with Popular Lesions and Retinoblastoma. Activated PKN1 stimulates transcription of the AR (androgen receptor)-regulated genes KLK2 and KLK3. Gene ontology annotations associated with this gene include DNA-bound transcription factor activity and transcription factor binding. An important homolog of this gene is HDAC2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.9 ★★★★★
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Product Reviews
★★★★★ 5
Doesnt burn and works wonders
Size: 6 Fl Oz (Pack of 1)
Works and light weight traveling
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 17, 2025
★★★★★ 5
BEST sunscreen! No greasy/sticky residue!
Size: 6 Fl Oz (Pack of 2), Style: Lotion
I HATE putting on sunscreen! They’re always greasy yet somehow leave your skin feeling sticky at the same time (especially the spray on kinds)?! Most of them have a super strong smell, and even if it’s a good smell it’s so strong it ends up giving me a headache. A couple years ago Coppertone came out with a whipped sunscreen that I was borderline obsessed with! It changed my life when it came to the dread of putting on sunscreen. It was moisturizing, but not greasy. It left not a hint of stickiness, and it didn’t not have an overwhelming smell! I was ecstatic! I had it set to auto deliver the double pack all summer long! My whole family and extended family also loved it! Well, the very next summer they discontinued it and my family and I (especially) we’re devastated! They didn’t even come out with something similar. It was just gone! Since then I’ve been searching for any kind of comparable sunscreen to no avail! I’ve tried them all! Then, about a month ago I found this one!! This is it!! It’s only as good as the coppertone whipped, but in some ways it’s better!! It’s not greasy or sticky at all. It spreads easily and has the softest, lightest, most amazing smell as well! It feels like I’m putting on my favorite lotion! Plus, no one in my family has gotten the slightest bit burned! Even my beautiful porcelain skinned 4 year old daughter who you have to drag off the beach or out of the pool when the sun goes down because she thinks she’s a mermaid! 🤣 I have nothing bad to say about this sunscreen!! I have stocked up just in case…. But Please please please don’t discontinue it!! 🙏🏻🙏🏻
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Reviewed in the United States on July 19, 2022
★★★★★ 5
Better than Shiseido for a fraction of the price
Size: 6 Fl Oz (Pack of 2), Style: Lotion
I bought this sunscreen for a trip to Florida but now it's my everyday sunscreen. My job includes driving around to body shops so my hands get a lot of sun, and I'm too embarrassed to wear driving gloves. I was using Shiseido's clear sunscreen stick (also SPF 50+) but had a slightly noticeable tan line at my wrist, both from my jacket and my watch so I wasn't sure if it was really working, despite the good reviews (and $$). This Banana Boat sunscreen feels so good that I decided to try it out for my hands every day, and it's fantastic. Smells good, not greasy at all it soaks right in, and the best part is my tan line is GONE. 10/10 please don't ever discontinue or change the formula.
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Reviewed in the United States on December 4, 2025
★★★★★ 5
The best sunblock I've found!
Size: 6 Fl Oz (Pack of 2), Style: Lotion
I absolutely love this sunblock. I have sensory issues with any type of lotion and sunblock, but this stuff is incredible. It really is so lightweight that once it fully soaks in (which only take a few minutes) it feels as if there's nothing on my skin. This is huge for me, cause I just can't handle the feeling of any other sunblocks I've tried, even some more expensive brands. On top of all that, it definitely protects my skin from the sun, doesn't leave a white cast, and doesn't feel greasy while applying it.
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Reviewed in the United States on April 12, 2026
★★★★★ 4
My favorite sunscreen for being active outside
Size: 6 Fl Oz (Pack of 2), Style: Lotion
I bought this last summer and now it's my go-to. I love it. It absorbs quickly, is very easy to just smear on, and has a nice, very light scent. I wear it when I go running and I sweat like a maniac so that tells you something.
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Reviewed in the United States on May 6, 2026
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