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Description
Histone H3 (acetyl K4 + K9 + K18) Rabbit polyclonal antibodyProduct Specification Host Rabbit Synonyms H3K4 9 18ac Immunogen Synthetic Peptide Location Nucleus Accession P68431 Antibody Type Polyclonal antibody Isotype IgG Application WB, IHC P, IF, ChIP Reactivity Hu, Ms, Rt Predicted Reactivity Fu, Pl, Ys, SeUr, Dr, C. el, Hm, Or, Bv, Xe Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300
Product Specification
| Host | Rabbit |
| Synonyms | H3K4/9/18ac |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, IF, ChIP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| IHC-P | 1:500 | |
| IF | 1:500 | |
| ChIP | 1:20~1:50 |
Background
Acetylated histones, octameric proteins that organize chromatin into nucleosomes, the basic structural unit of the chromosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription.
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