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Description
Human CFHR1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Complement Factor H Related Protein 1 (CFHR1). After incubation and washing, the color is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Complement Factor H Related Protein 1 (CFHR1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Complement Factor H Related Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Complement factor H-related protein 1 is encoded by the CFHR1 gene. It binds to the Pseudomonas aeruginosa elongation factor Tuf and is activated by the protease. Tuf functions as a virulence factor by acquiring host proteins onto the pathogen surface, controlling complement, and promoting tissue invasion. Mutations in this gene are associated with an increased risk of atypical hemolytic uremic syndrome. Diseases associated with CFHR1 include hemolytic uremic syndrome, atypical 1, and age-related macular degeneration 1. An important homologue of this gene is CFHR2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.4 ★★★★★
Based on 80 reviews
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Product Reviews
★★★★★ 2
Ordered new, got it used
Format: Paperback
Paid for a new book. It arrived very clearly used. It is in decent condition but it is frustrating paying that much for something to arrive used with damage.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 17, 2026
★★★★★ 5
Excellent and Necessary!
Format: Kindle
Adding this book to the impactful list in my life! Very well written and easy to read. Thank you John Mark Comer.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 2, 2026
★★★★★ 5
A much needed quench to the thirst of our societies internal struggles.
Format: Hardcover
The Ruthless Elimination of Hurry:
This book is a refreshing surge of truth and wisdom, full of profound teachings from a heart posture of love for those of us suffering from modern day hyper-culture. Thank you for the great read, John Mark Comer.
I would highly recommend to others to read this book: To slowly and fully read this book.
After the first chapter, I was confronted with so many modern day realities that have become the new daily norms. Those of course brought forth personal conviction and a new hope for the change that would be to come! It was hard to believe that the entire book would be so filling - BUT IT WAS.
The Ruthless Elimination of Hurry makes an articulate yet simple stride to sober today’s society to the truth. The truth that the generalized hurried, stressed out, and “got no time” culture we’ve created, is bringing us death. Not life.
JMC leads a church that I regularly visit. I do not come because I have an overflowing abundance of time and want to go to church twice on Sundays. No, I go because everytime I attend Bridgetown Church, I am filled with deeper insight into God’s word and such joy and peace! Why joy and peace you may ask? Because our God is a God of JOY and PEACE. When you get to know him and his truths are spoken to us and over our life we begin to know our Father and find joy, rest, and many other fruits in him. JMC and his pastoral team are doing something beautifully radical in today’s society - Choosing Jesus’ rhythm over today’s western hyper obsessed, and greed driven culture.
I began reading this book during a month of my life that I felt like I was spiritually under warfare and was having a hard time not just spiritually, but also emotionally and physically. I began reading the Ruthless Elimination of
Hurry because I have read all of JMC’s books and I trust that his writings would be only an encouragement for me. This book is full of life giving knowledge that he has collected through his discipline to the way, reading other authors, historical and present, that too were trying to practice the way, and oh yeah - where they got it all - Jesus, the man teaching us all the way! This read was a leveling reminder of my discipleship to Jesus and how to step back into his beautifully easy rhythm that is, no doubt, in opposition to the rhythm of current society, social media, and today’s lure of entertainment. I am twisting and turning my way back into the path God has laid so lovingly before us to stride beside him. & just like that! I am back to the fruit of the spirit spilling joy and love out of me!
I cried once in this novel. As I read the last three pages. Please listen why - Because JMC so graciously and intentionally laid in front of us Jesus’ teachings in a language we all follow, a way to heal our souls, bring families back together, unite friendships, & ultimately live life to the fullest. I was filled with a passionate excitement to again believe that WE CAN live in the way of Jesus, walking in his slow and easy footsteps.
In my opinion, this is John Mark Comer’s best novel yet. I feel as though we have a modern day apostle helping us to disciple under Jesus. Ready and willing to teach those who choose to listen and leading the masses into the loving way of Jesus.
I bought this book for myself. & then I bought a couple for a couple close friends. I imagine I will continue as I see the need in many struggling in a chaotic and over crammed life. This is a refreshing reminder to step back into the path less traveled. I encourage you to read it and see what kind of fruit these practices can bear in your life.
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Reviewed in the United States on November 9, 2019
★★★★★ 5
Slowdown. Chill. Enjoy life.
Format: Hardcover
Very simple read with great content for slowing down.
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Definitely a really good book to read
Format: Hardcover
So far an amazing book I haven’t finished it, but I’m so into it and I know for a fact it’s gonna be good from the beginning to the end. It does make me reflect on my life, and it has opened my eyes to a lot of things regarding being in a hurry, and how if the enemy can’t get us to sin, he will make us busy. Must read.
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Reviewed in the United States on April 13, 2026