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Description
Human SLC39A4 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against zinc transporter ZIP4 (SLC39A4). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of zinc transporter ZIP4 (SLC39A4) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Zinc transporter ZIP4 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Solute carrier family 39, member 4 (SLC39A4), also known as zinc transporter ZIP4 (ZIP4), is encoded by the SLC39A4 gene. It is a transmembrane protein that has been associated with acrodermatitis enteropathica. As a zinc transporter, it plays an important role in cellular zinc homeostasis. This gene encodes a member of the zinc/iron regulated transporter-like protein (ZIP) family. The encoded protein is localized to the cell membrane and is required for intestinal zinc uptake. Mutations in this gene cause acrodermatitis enteropathica. Multiple transcript variants encoding different isoforms have been identified for this gene. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.4 ★★★★★
Based on 430 reviews
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★★★★★ 4
The Ordinary Hyaluronic Acid 2% + B5 (with Ceramides)
Size: 1 Fl Oz (Pack of 1)
The Ordinary’s Hyaluronic Acid 2% + B5 with added ceramides is a hydration powerhouse that delivers noticeable moisture and placid comfort to dry, dehydrated, or combination skin. Hyaluronic acid draws water into the skin’s surface layers, helping skin feel plump, soft, and smooth, while vitamin B5 supports enhanced surface hydration and recovery.
Adding ceramides is a smart touch because they help reinforce the skin barrier, locking in moisture and preventing water loss throughout the day. The result is a formula that doesn’t just sit on the surface — it helps improve the skin’s resilience over time.
The texture is lightweight and gel-like, absorbing quickly without leaving a sticky or heavy feel, making it easy to layer under serums, moisturizers, and sunscreen. It works beautifully in both morning and evening routines.
After consistent use, many users notice better overall hydration, smoother fine lines caused by dryness, and a more comfortable complexion — especially during dry seasons or in air-conditioned environments.
Pros:
✔ Deep hydration that lasts
✔ Lightweight and fast-absorbing
✔ Great paired with other products (moisturizers / creams)
✔ Ceramides help support the skin barrier
Cons:
✖ Not a direct treatment for acne or pigmentation
✖ Effects build gradually with consistent use
Overall: A very effective and versatile hydrator that’s easy to use and works well for most skin types. Particularly great if you want lasting moisture and a smoother, healthier-feeling complexion.
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Reviewed in the United States on January 27, 2026
★★★★★ 5
Amazing product - instant smoothness
Size: 1 Fl Oz (Pack of 1)
I've only had this product for the last several days, but, so far, it's AMAZING. I've tried hyaluronic products before, because everyone says that you are supposed to for moisture, but, honestly, I was always underwhelmed. They were thin and did nothing. So, despite this one having great reviews, I was not really expecting anything, but the price was right.
But this product....although liquid and clear, it's got a slight thickness to it, and spreads on very smooth. You only need a little bit to cover your entire face. Despite the slight thickness, it's not gooey or sticky at all, and it dries incredibly fast. In fact, if you don't spread it around onto your face quickly enough, it's already drying. There is no real smell to it. And, as soon as it dries onto your face, your skin feels incredibly smooth. Maybe it wouldn't if you had a significant dry skin problem to begin with, but for regular, maturing skin, it leaves skin baby smooth. And it lasts all day, and my skin looks better.
I have very sensitive skin, and most things on my face eventually make my eyes water (even gentle facial moisturizers and sunscreens), but, so far, I haven't had an issue, and, because it's been a few days already, maybe I won't. Doesn't really feel like I'm wearing anything, and isn't a problem if I want to layer it with something else. I do wash it off at night, and can tell that something is still on my face even at the end of the day because it's a little slippery when I wash it. So it has staying power.
For the price, I would definitely consider trying this product for moisturizing, and instantly smoother and softer skin.
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Reviewed in the United States on April 3, 2026
★★★★★ 5
plumping hydration with no greasiness or oiliness
Size: 1 Fl Oz (Pack of 1)
Its featherweight and melds with the skin in an instant. It plumps without any residue or "slip" on the skin. I have normal skin that tends toward slight dehydration and I use adapalene and the Ordinary 2% Salicylic acid anhydrous for my sebaceous hyperplasia. Because of the sebaceous hyperplasia and the heat and extreme humidity where I live, I cannot use anything with oils or dimethicone or anything even remotely occlusive. This serum is a great way for me to get appropriate hydration without exacerbating those enlarged oil glands or making my face sticky and oily. I dont need my face sealed, a humectant like this pulls plenty of hydration into my skin and I dont lose it to the air because of the ambient humidity. Its pretty perfect for me. It also has kept my barrier intact while alternating the adapalene and salicylic acid. Its a huge bang for the buck, I mean the price point is unbeatable.
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Reviewed in the United States on May 11, 2026
★★★★★ 5
Great lights
Size: 2PCS 23''x5''
Very nice lights. They look really cool, and were well packaged. If you are looking for a fun accessory for your vehicle that makes people do a double take... then this is for you!
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Fun
Size: 11''x5''
Works great
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Reviewed in the United States on June 1, 2026